Six-Session Hands-On Program

The Medical Analysis Department at the Faculty of Applied Science, Tishk International University, successfully completed a comprehensive six-session hands-on training course on molecular biology techniques, designed to integrate theoretical knowledge with laboratory practice.

The program guided students through the complete molecular workflow — from theoretical lectures to DNA extraction, PCR amplification, gel electrophoresis, visualization, and documentation.


Session-by-Session Breakdown

Session Date Format Content
Session 1 January 25, 2026 Theoretical Course outline, laboratory safety, fundamental principles of molecular biology
Session 2 January 26, 2026 Practical Genomic DNA extraction from human samples (sample processing, cell lysis, DNA isolation, purification)
Session 3 February 1, 2026 Theoretical Polymerase Chain Reaction (PCR): mechanism, essential components, types of PCR techniques
Session 4 February 2, 2026 Practical PCR amplification: master mix preparation, reagent loading, thermocycler operation
Session 5 February 8, 2026 Theoretical Agarose gel electrophoresis: gel preparation, concentration differences, DNA ladders, visualization techniques
Session 6 February 9, 2026 Practical Gel electrophoresis of PCR products: sample loading, electrophoretic separation, UV transillumination, gel documentation

Supervision and Instruction

The practical sessions were supervised by:

Name Role
Dr. Sami Mamand Head of the Medical Analysis Department
Dr. Galawezh Obaid Academic staff at TIU
Dr. Jaafaru Sani Academic staff at TIU

Emphasis was placed on:

  • Sterility throughout all procedures

  • Correct laboratory handling techniques

  • Safe operation of molecular biology equipment


Learning Outcomes

Through this structured program, participants successfully:

  • Performed genomic DNA extraction from human samples

  • Conducted PCR amplification using their extracted DNA

  • Prepared and performed agarose gel electrophoresis

  • Visualized and documented amplified DNA fragments using UV transillumination

  • Completed the full molecular biology workflow from sample to result


Department Commitment

This training course reflects the department’s ongoing commitment to:

  • Strengthening practical and academic excellence

  • Integrating theoretical knowledge with laboratory practice

  • Equipping students with essential molecular biology skills

Keywords: Molecular Biology Training, Hands-On Course, DNA Extraction, PCR Amplification, Gel Electrophoresis, Medical Analysis Department, Dr. Sami Mamand, Dr. Galawezh Obaid, Dr. Jaafaru Sani, TIU Applied Science, Laboratory Skills