Molecular Biology Laboratory (MBL)

The Molecular Biology Laboratory (MBL) provides an advanced environment for molecular biology research, scientific innovation, and academic collaboration. The laboratory supports researchers, students, and academic staff with modern technologies and facilities for high-quality molecular analyses and experimental work.

MBL offers a range of molecular techniques, including Conventional PCR, Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), and Droplet Digital PCR (ddPCR), using advanced systems from Bio-Rad Laboratories.

One of the key strengths of MBL is the Bio-Rad QX200 ddPCR System, which enables highly sensitive and precise nucleic acid quantification for various research and diagnostic applications.

Through its modern facilities and research-focused environment, MBL aims to support scientific progress and contribute to advancements in molecular biology and biomedical sciences.

Molecular Technologies Available at MBL

 

 

Conventional PCR

Conventional PCR is a widely used molecular technique for amplifying
specific DNA sequences. It is commonly applied in genetic analysis,
pathogen detection, and molecular research.

 

Real-Time PCR (qPCR)

Real-Time PCR, also known as quantitative PCR (qPCR), allows the monitoring and quantification of DNA amplification in real time. This technique is highly sensitive and is widely used in gene expression studies, infectious disease detection, and molecular diagnostics.

 

 

 

Reverse Transcription PCR (RT-PCR)

RT-PCR is used to convert RNA into complementary DNA (cDNA) before amplification.
This method is essential for studying RNA viruses, gene expression,
and transcriptomic analysis.

 

 

 

Droplet Digital PCR (ddPCR)

Droplet Digital PCR (ddPCR) is an advanced form of PCR technology that enables
absolute quantification of nucleic acids with exceptional sensitivity
and precision, without the need for standard curves.

The Bio-Rad QX200 ddPCR System partitions samples into thousands
of nanoliter-sized droplets, allowing individual PCR reactions to occur within
each droplet. This approach provides highly accurate detection and quantification
of target DNA or RNA molecules.

Key Advantages of ddPCR
  • Absolute quantification without standard curves
  • Higher precision and sensitivity compared to conventional PCR methods
  • Detection of rare mutations and low-abundance targets
  • Improved reproducibility and reliability of results

Common Applications of ddPCR

  • Cancer mutation detection and liquid biopsy analysis
  • Viral load quantification (e.g., HIV, COVID-19)
  • Gene expression analysis
  • Copy number variation (CNV) studies
  • Rare mutation detection
  • GMO detection and molecular diagnostics